A new method of measuring aqueous humor flow and corneal endothelial permeability to fluorescein using a fluorophotometry nomogram is described. This method is compared to four other methods, two of which have been described previously. All five methods were carried out simultaneously in a group of 20 normal human subjects. All methods give closely comparable results in most of the eyes tested. A tabular comparison of the results of the nomographic method and the published results of other investigators, which are in good agreement, is given. The relative advantages and disadvantages of the nomographic technique are discussed.
15 Figures and Tables
Fig. 1. Kinetic model of fluorescein illustrating analogy to commonly used single-dose pharmacokinetic model.
Fig. 10. Fluorescein kinetics in eye after iontophoresis into central cornea, expressed as fraction of initial dose, illustrating theoretical effects of abnormal aqueous humor flow and abnormal corneal endothelial permeability.
Fig. 2. Fluorophotometry data reduction method 1 (Jones and Maurice,5 method 1).
Fig. 3. Fluorophotometry data reduction method 2 (Jones and Maurice,5 method 2).
Fig. 4. Fluorophotometiy data reduction method 3 (Wagner and Nelson1
Fig. 5. Fluorophotometiy data reduction methods 4 and 5 (nomographic and successive approximation methods).
Fig. 6. Fluorophotometry nomogram, calculated for eye with anterior chamber volume of 100 fx\.
Fig. 7. Fluorophotometry nomogram, calculated for eye with anterior chamber volume of 200 /Hi.
Fig. 8. Fluorophotometry nomogram, calculated for eye with anterior chamber volume of 300 ju.1.
Fig. 9. Fluorescein data of subject MLL-1, carried out to 9 hr. Dots are data values; curves are the theoretical values of total mass and anterior chamber concentration derived from the results in this subject of method 5.
Table IA. Measured fluorescein data of 20 normal human subjects*
Table IB. Calculated transfer coefficients and aqueous humor flow, 20 normal human subjects, using five methods of data analysis
Table II. Corneal endothelial permeability to fluorescein in the normal human eye
Table IV. Table of comparison of methods 2 and 4
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